Coding
PTC Globin

Part:BBa_K2083011:Design

Designed by: Virginia Massa   Group: iGEM16_WPI_Worcester   (2016-10-14)


Human Beta Globin With a Premature Termination Codon Fused to GFP Reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We performed codon optimization to removed any illegal restriction sites.


Source

This a human beta globin sequence with the first two exons fused. GFP is fused to the end of the end of the sequence.

References